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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX102048 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX102048, RRID:AB_1241254
- Product name
- TRIM27 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Comparison of an expanded ataxia interactome with patient medical records reveals a relationship between macular degeneration and ataxia.
Kahle JJ, Gulbahce N, Shaw CA, Lim J, Hill DE, Barabási AL, Zoghbi HY
Human molecular genetics 2011 Feb 1;20(3):510-27
Human molecular genetics 2011 Feb 1;20(3):510-27
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western Blot analysis of TRIM27 expression in transfected 293T cell line by TRIM27 polyclonal antibody. A: Non-transfected lysate. B: TRIM27 transfected lysate. 7.5% SDS PAGEGTX102048 diluted at 1:500
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TRIM27 antibody detects TRIM27 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM27 antibody (GTX102048) diluted by 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TRIM27 antibody detects TRIM27 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with TRIM27 antibody (GTX102048) diluted at a dilution of 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 £gg) was separated by 10% SDS-PAGE, and the membrane was blotted with TRIM27 antibody (GTX102048) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TRIM27(GTX102048) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of TRIM27 protein from Jurkat whole cell extracts using 5 £gg of TRIM27 antibody (GTX102048).Western blot analysis was performed using TRIM27 antibody (GTX102048).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
