Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [14]
- Other assay [1]
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Validation data
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- Product number
- 12205-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRIM27 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: EQARAELLS LTQMEREKIV WEFEQLYHSL KEHEYRLLAR LEELDLAIYN SINGAITQFS CNISHLSSLI AQLEEKQQQP TRELLQDIGD TLSRAERIRI PEPWITPPDL QEKIHIFAQK CLFLTESLKQ FTEKMQSDME KIQELREAQL YSVDVTLDPD TAYPSLILSD NLRQVRYSYL QQDLPDNPER FNLFPCVLGS PCFIAGRHYW EVEVGDKAKW TIGVCEDSVC RKGGVTSAPQ NGFWAVSLWY GKEYWALTSP MTALPLRTPL QRVGIFLDYD AGEVSFYNVT ERCHTFTFSH ATFCGPVRPY FSLSYSGGKS AAPLIICPMS GIDGFSGHVG NHGHSMETSP (165-514 aa encoded by BC013580)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 12205-1-AP (TRIM27 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 12205-1-AP (TRIM27 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of TRIM27 antibody (12205-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM27 transfected Jurkat cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human testis tissue were subjected to SDS PAGE followed by western blot with 12205-1-AP (TRIM27 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Jurkat cells were subjected to SDS PAGE followed by western blot with 12205-1-AP (TRIM27 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 12205-1-AP (TRIM27 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HeLa cells using 12205-1-AP (TRIM27 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 12205-1-AP (TRIM27 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin using 12205-1-AP (TRIM27 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen using 12205-1-AP (TRIM27 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human spleen using 12205-1-AP (TRIM27 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human heart using 12205-1-AP (TRIM27 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 12205-1-AP (TRIM27 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney using 12205-1-AP (TRIM27 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta using 12205-1-AP (TRIM27 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human placenta using 12205-1-AP (TRIM27 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 12205-1-AP (TRIM27 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 12205-1-AP (TRIM27 antibody) at dilution of 1:100 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin using 12205-1-AP (TRIM27 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using 12205-1-AP (TRIM27 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using (Product # 12205-1-AP) (TRIM27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-TRIM27 (IP:12205-1-AP, 4ug; Detection:12205-1-AP 1:1000) with mouse testis tissue lysate 4000ug.