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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-27619 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRIM27 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: IMR32.
- Concentration
- 0.13 mg/mL
Submitted references TRIM27 promotes IL-6-induced proliferation and inflammation factor production by activating STAT3 signaling in HaCaT cells.
Miao X, Xiang Y, Mao W, Chen Y, Li Q, Fan B
American journal of physiology. Cell physiology 2020 Feb 1;318(2):C272-C281
American journal of physiology. Cell physiology 2020 Feb 1;318(2):C272-C281
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TRIM27 using 30 µg of HeLa lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a TRIM27 polyclonal antibody (Product # PA5-27619) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TRIM27 was performed by separating 30 µg of whole cell extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a TRIM27 Polyclonal Antibody (Product # PA5-27619) at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of TRIM27 in paraformaldehyde-fixed HeLa cells using a TRIM27 polyclonal antibody (Product # PA5-27619) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using ret finger protein (Product # PA5-27619) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
