Explore
Validate
Learn
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB2186 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Carbonic Anhydrase IV/CA4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Carbonic Anhydrase IV/CA4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant human (rh) CA1, rhCA2, and recombinant mouse CA4 is observed. Does not cross-react with rhCA3, 8, 9, 10, 12, 13, or 14.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P22748- Isotype
- IgG
- Antibody clone number
- 310413
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Membrane-anchored carbonic anhydrase IV interacts with monocarboxylate transporters via their chaperones CD147 and GP70.
Intracellular and extracellular carbonic anhydrases cooperate non-enzymatically to enhance activity of monocarboxylate transporters.
Transport activity of the high-affinity monocarboxylate transporter MCT2 is enhanced by extracellular carbonic anhydrase IV but not by intracellular carbonic anhydrase II.
Studies on bicarbonate transporters and carbonic anhydrase in porcine nonpigmented ciliary epithelium.
Forero-Quintero LS, Ames S, Schneider HP, Thyssen A, Boone CD, Andring JT, McKenna R, Casey JR, Deitmer JW, Becker HM
The Journal of biological chemistry 2019 Jan 11;294(2):593-607
The Journal of biological chemistry 2019 Jan 11;294(2):593-607
Intracellular and extracellular carbonic anhydrases cooperate non-enzymatically to enhance activity of monocarboxylate transporters.
Klier M, Andes FT, Deitmer JW, Becker HM
The Journal of biological chemistry 2014 Jan 31;289(5):2765-75
The Journal of biological chemistry 2014 Jan 31;289(5):2765-75
Transport activity of the high-affinity monocarboxylate transporter MCT2 is enhanced by extracellular carbonic anhydrase IV but not by intracellular carbonic anhydrase II.
Klier M, Schüler C, Halestrap AP, Sly WS, Deitmer JW, Becker HM
The Journal of biological chemistry 2011 Aug 5;286(31):27781-91
The Journal of biological chemistry 2011 Aug 5;286(31):27781-91
Studies on bicarbonate transporters and carbonic anhydrase in porcine nonpigmented ciliary epithelium.
Shahidullah M, To CH, Pelis RM, Delamere NA
Investigative ophthalmology & visual science 2009 Apr;50(4):1791-800
Investigative ophthalmology & visual science 2009 Apr;50(4):1791-800
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Carbonic Anhydrase IV/CA4 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human lung tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Carbonic Anhydrase IV/CA4 Monoclonal Antibody (Catalog # MAB2186) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Carbonic Anhydrase IV/CA4 at approximately 35 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
