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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- GTX100438 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100438, RRID:AB_1949569
- Product name
- TRAP antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Odontoclastogenesis of mouse papilla-derived MDPC-23 cells induced by lipopolysaccharide.
Tensile force on human macrophage cells promotes osteoclastogenesis through receptor activator of nuclear factor κB ligand induction.
The S349T mutation of SQSTM1 links Keap1/Nrf2 signalling to Paget's disease of bone.
Li C, Qi WT, Jiang HW
International endodontic journal 2018 Feb;51 Suppl 2:e115-e124
International endodontic journal 2018 Feb;51 Suppl 2:e115-e124
Tensile force on human macrophage cells promotes osteoclastogenesis through receptor activator of nuclear factor κB ligand induction.
Kao CT, Huang TH, Fang HY, Chen YW, Chien CF, Shie MY, Yeh CH
Journal of bone and mineral metabolism 2016 Jul;34(4):406-16
Journal of bone and mineral metabolism 2016 Jul;34(4):406-16
The S349T mutation of SQSTM1 links Keap1/Nrf2 signalling to Paget's disease of bone.
Wright T, Rea SL, Goode A, Bennett AJ, Ratajczak T, Long JE, Searle MS, Goldring CE, Park BK, Copple IM, Layfield R
Bone 2013 Feb;52(2):699-706
Bone 2013 Feb;52(2):699-706
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A:NIH-3T310% SDS PAGE GTX100438 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ?g whole cell lysate)A:A431(GTX27909)B:MOLT4 (GTX27912)12% SDS PAGEGTX100438 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TRAP antibody detects TRAP protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 10% SDS-PAGE, and the membrane was blotted with TRAP antibody (GTX100438) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TRAP antibody detects ACP5 protein at cytosol on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine tissue. TRAP antibody (GTX100438) dilution: 1:500.
