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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF3948 - Provider product page
- Provider
- R&D Systems
- Product name
- Human TRACP/PAP/ACP5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human TRACP/PAP/ACP5 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P13686- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human TRACP/PAP/ACP5 by Western Blot. Western blot shows lysates of human lung tissue, SK-Mel-28 human malignant melanoma cell line and Recombinant Human TRACP/PAP/ACP5 (Catalog # 3948-AP). PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human TRACP/PAP/ACP5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3948) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for TRACP/PAP/ACP5 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
