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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NBP1-77935 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77935, RRID:AB_11010189
- Product name
- Rabbit Polyclonal ANKRD26 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is directed against mouse Ankrd26 protein. A BLAST analysis was used to suggest cross reactivity with Ankrd26 protein.
- Reactivity
- Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.48 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: ANKRD26 Antibody [NBP1-77935] - shows detection of a band at ~81 kDa corresponding to mouse Ankrd26 protein. Lane 1 Blank, Lane 2 MES cell lysate - 80 ug, Lane 3 MES cell lysate - 40 ug, Lane 4 293T-ANKRD26 transfected cell lysate - 20 ug, Lane 5 control 293T cell lysate - 20 ug, Lane 6 BSA-ANKRD26 conjugate 20 ng, Lane 7 BSA - 500 ng, Lane 8 BSA - 100 ng, Lane 9 BSA 20 ng and Lane 10 Protein standards. Detection of endogenous Ankrd26 protein in MES cell lysates may occur when detection methods with higher sensitivity are used. Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1,000 followed by detection using ALP conjugated Gt-a-Rabbit IgG diluted to 1:3,000. Size estimation was made by comparison to prestained MW markers as indicated.
