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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- PAB10090 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10090, RRID:AB_1672107
- Product name
- Ankrd26 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to amino acids 1-15 of mouse Ankrd26.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Vial size
- 100 µg
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using Ankrd26 polyclonal antibody (Cat # PAB10090) shows detection of a band at ~81 KDa corresponding to mouse Ankrd26 protein.Lane 1 Blank, Lane 2 MES cell lysate - 80 ug, Lane 3MES cell lysate-40 ug, Lane 4 293T-Ankrd26 transfected cell lysate-20 ug, Lane 5 control 293T celllysate-20 ug, Lane 6 BSA-Ankrd26 conjugate-20ng, Lane 7 BSA-500 ng, Lane 8 BSA-100 ng, Lane 9 BSA-20 ng.Detection of endogenous Ankrd26 protein in MES cell lysates may occur when detection methods with higher sensitivity are used.Proteins were separated by SDS-PAGE, transferred to nitrocellulose, and probed withthe primary antibody diluted to 1:1,000 followed by detection using ALP conjugated Gt-a-Rabbit IgG diluted to 1:3,000.Sizeestimation was made by comparison to prestained MW markers as indicated.Personal Communication.Ira Pastan, NIH, CCR, Bethesda, MD.
