AP09309PU-N

antibody from Acris Antibodies GmbH

Targeting: ANKRD26 KIAA1074, THC2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

AP09309PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP09309PU-N, RRID:AB_2035122

Product name

anti ANKRD26 (1-15)

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to amino acids 1-15 of mouse Ankrd26 protein

Reactivity

Human, Mouse, Rat, Bovine

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.48 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot using affinity purified anti-Ankrd26 antibody shows detection of a band at ~81 kDa corresponding to mouse Ankrd26 protein. Lane 1 Blank, Lane 2 MES cell lysate - 80 µg, Lane 3 MES cell lysate - 40 µg, Lane 4 293T-ANKRD26 transfected cell lysate - 20 µg, Lane 5 control 293T cell lysate - 20 µg, Lane 6 BSA-ANKRD26 conjugate - 20 ng, Lane 7 BSA - 500 ng, Lane 8 BSA - 100 ng, Lane 9 BSA - 20 ng and Lane 10 Protein standards. Detection of endogenous Ankrd26 protein in MES cell lysates may occur when detection methods with higher sensitivity are used. Proteins were separated by SDSPAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1,000 followed by detection using ALP conjugated Gt-a-Rabbit IgG diluted to 1:3,000. Size estimation was made by comparison to prestained MW markers as indicated.