Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- AP09309PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09309PU-N, RRID:AB_2035122
- Product name
- anti ANKRD26 (1-15)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide corresponding to amino acids 1-15 of mouse Ankrd26 protein
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.48 mg/ml (by UV absorbance at 280 nm)
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blot using affinity purified anti-Ankrd26 antibody shows detection of a band at ~81 kDa corresponding to mouse Ankrd26 protein. Lane 1 Blank, Lane 2 MES cell lysate - 80 µg, Lane 3 MES cell lysate - 40 µg, Lane 4 293T-ANKRD26 transfected cell lysate - 20 µg, Lane 5 control 293T cell lysate - 20 µg, Lane 6 BSA-ANKRD26 conjugate - 20 ng, Lane 7 BSA - 500 ng, Lane 8 BSA - 100 ng, Lane 9 BSA - 20 ng and Lane 10 Protein standards. Detection of endogenous Ankrd26 protein in MES cell lysates may occur when detection methods with higher sensitivity are used. Proteins were separated by SDSPAGE, transferred to nitrocellulose, and probed with the primary antibody diluted to 1:1,000 followed by detection using ALP conjugated Gt-a-Rabbit IgG diluted to 1:3,000. Size estimation was made by comparison to prestained MW markers as indicated.