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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [4]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-21339 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SEC13 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SEC13 protein isoform 1 using 30 µg of A) 293T (B) A431 and C) H1299 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a SEC13 protein isoform 1 polyclonal antibody (Product # PA5-21339) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using SEC13 Polyclonal Antibody (Product # PA5-21339). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SEC13 Polyclonal Antibody (Product # PA5-21339) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SEC13 protein isoform 1 showing staining in the vesicles of HepG2 cells. HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a SEC13 protein isoform 1 polyclonal antibody (Product # PA5-21339) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of SEC13 protein isoform 1 showing staining in the vesicles of HepG2 cells. HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a SEC13 protein isoform 1 polyclonal antibody (Product # PA5-21339) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SEC13 Polyclonal Antibody detects SEC13L1 protein at COP II vesicles by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SEC13L1 protein stained by SEC13 Polyclonal Antibody (Product # PA5-21339) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SEC13 Polyclonal Antibody detects SEC13L1 protein at COP II vesicles by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SEC13L1 protein stained by SEC13 Polyclonal Antibody (Product # PA5-21339) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using SEC13L1 (Product # PA5-21339) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SEC13L1 antibody immunoprecipitates SEC13L1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 µg whole cell lysate/extract of SEC13L1 protein expressing HepG2 cells B : Control with 2.5 µg of pre-immune rabbit IgG C : Immunoprecipitation of SEC13L1 by 2.5 µg of SEC13L1 antibody (Product # PA5-21339) 10% SDS-PAGE The immunoprecipitated SEC13L1 protein was detected by SEC13L1 antibody (Product # PA5-21339) diluted at 1:1,000. Anti-rabbit IgG (HRP) was used as a secondary reagent.
