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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- NBP1-33291 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-33291, RRID:AB_2186235
- Product name
- Rabbit Polyclonal SEC13 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SEC13 Antibody [NBP1-33291] - Sample (30 ug of whole cell lysate) A: 293T B: A431 C: H1299 10% SDS PAGE; antibody diluted at 1:1000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SEC13 Antibody [NBP1-33291] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of SEC13L1 protein expressing HepG2 cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of SEC13L1 by 2.5 ug of SEC13L1 antibody 10% SDS-PAGE The immunoprecipitated SEC13L1 protein was detected by SEC13L1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SEC13 Antibody [NBP1-33291] - Paraffin-embedded A549 xenograft, using antibody at 1:100 dilution.
