Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- MA1-721 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GRK1 Monoclonal Antibody (D11)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA1-721 detects G-protein-associated rhodopsin kinase 1a/b (GRK1a/b) from human and bovine tissues. This antibody does not cross-react with other G protein-coupled kinases. MA1-721 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation experiments. By Western blot, this antibody detects an ~60 kDa protein representing GRK 1a/b. MA1-721 detects both in vitro expressed splice variants by Western blot but was not able to detect significant amounts of GRK1b from human retina. Immunofluorescence staining of GRK1a/b in bovine retina with MA1-721 results in intense staining primarily in cone outer segments, but also in rod outer segments. Weak staining is observed in somata and synaptic terminals of cones and inner segments of rods. The MA1-721 immunogen is full length human GRK1a. This antibody recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b.
- Reactivity
- Human, Mouse, Bovine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- D11
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Acute and Protracted Cell Death in Light-Induced Retinal Degeneration in the Canine Model of Rhodopsin Autosomal Dominant Retinitis Pigmentosa.
Phosphorylation of G protein-coupled receptor kinase 1 (GRK1) is regulated by light but independent of phototransduction in rod photoreceptors.
GAP-independent termination of photoreceptor light response by excess gamma subunit of the cGMP-phosphodiesterase.
A short, highly active photoreceptor-specific enhancer/promoter region upstream of the human rhodopsin kinase gene.
GRK1-dependent phosphorylation of S and M opsins and their binding to cone arrestin during cone phototransduction in the mouse retina.
Sudharsan R, Simone KM, Anderson NP, Aguirre GD, Beltran WA
Investigative ophthalmology & visual science 2017 Jan 1;58(1):270-281
Investigative ophthalmology & visual science 2017 Jan 1;58(1):270-281
Phosphorylation of G protein-coupled receptor kinase 1 (GRK1) is regulated by light but independent of phototransduction in rod photoreceptors.
Osawa S, Jo R, Xiong Y, Reidel B, Tserentsoodol N, Arshavsky VY, Iuvone PM, Weiss ER
The Journal of biological chemistry 2011 Jun 10;286(23):20923-9
The Journal of biological chemistry 2011 Jun 10;286(23):20923-9
GAP-independent termination of photoreceptor light response by excess gamma subunit of the cGMP-phosphodiesterase.
Tsang SH, Woodruff ML, Chen CK, Yamashita CY, Cilluffo MC, Rao AL, Farber DB, Fain GL
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Apr 26;26(17):4472-80
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Apr 26;26(17):4472-80
A short, highly active photoreceptor-specific enhancer/promoter region upstream of the human rhodopsin kinase gene.
Young JE, Vogt T, Gross KW, Khani SC
Investigative ophthalmology & visual science 2003 Sep;44(9):4076-85
Investigative ophthalmology & visual science 2003 Sep;44(9):4076-85
GRK1-dependent phosphorylation of S and M opsins and their binding to cone arrestin during cone phototransduction in the mouse retina.
Zhu X, Brown B, Li A, Mears AJ, Swaroop A, Craft CM
The Journal of neuroscience : the official journal of the Society for Neuroscience 2003 Jul 9;23(14):6152-60
The Journal of neuroscience : the official journal of the Society for Neuroscience 2003 Jul 9;23(14):6152-60
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Rhodopsin Kinase 1a/b (green) in human cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Rhodopsin Kinase 1a/b Monclonal Antibody (D11) (Product # MA1-721) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Rhodopsin Kinase 1a/b (green) in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Rhodopsin Kinase 1a/b Monclonal Antibody (D11) (Product # MA1-721) at a dilution of 1:200 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Rhodopsin Kinase 1a/b (green) in murine cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Rhodopsin Kinase 1a/b Monclonal Antibody (D11) (Product # MA1-721) at a dilution of 1:200 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunolocalization of GRK1a/b in bovine retina using Product # MA1-721.