Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- ABIN952269 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Ubiquitin D (UBD) (C-Term), (AA 123-153) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 123-153 amino acids from the C-terminal region of human FAT10
- Description
- Affinity Chromatography on Protein A
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Epitope
- C-Term,AA 123-153
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Examination of genetic polymorphisms in newborns for signatures of sex-specific prenatal selection.
A regulatory single nucleotide polymorphism in the ubiquitin D gene associated with celiac disease.
The ubiquitin-like protein FAT10 mediates NF-kappaB activation.
Ucisik-Akkaya E, Davis CF, Do TN, Morrison BA, Stemmer SM, Amadio WJ, Dorak MT
Molecular human reproduction 2010 Oct;16(10):770-7
Molecular human reproduction 2010 Oct;16(10):770-7
A regulatory single nucleotide polymorphism in the ubiquitin D gene associated with celiac disease.
Castellanos-Rubio A, Santin I, Irastorza I, Sanchez-Valverde F, CastaƱo L, Vitoria JC, Bilbao JR
Human immunology 2010 Jan;71(1):96-9
Human immunology 2010 Jan;71(1):96-9
The ubiquitin-like protein FAT10 mediates NF-kappaB activation.
Gong P, Canaan A, Wang B, Leventhal J, Snyder A, Nair V, Cohen CD, Kretzler M, D'Agati V, Weissman S, Ross MJ
Journal of the American Society of Nephrology : JASN 2010 Feb;21(2):316-26
Journal of the American Society of Nephrology : JASN 2010 Feb;21(2):316-26
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of FAT10 Antibody (C-term) Cat.-No AP51613PU-N in mouse liver tissue lysates (35ug/lane). This demonstrates the hFAT10 antibody detected the hFAT10 protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of FAT10 Antibody (C-term) Cat.-No AP51613PU-N with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin fixed and paraffin embedded human stomach carcinoma reacted with FAT10 Antibody (C-term) Cat.-No AP51613PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.