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LearnH00055741-M01
antibody from Novus Biologicals
Targeting: EDEM2
bA4204.1, C20orf31, C20orf49, FLJ10783
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [2]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00055741-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00055741-M01, RRID:AB_2096093
- Product name
- Mouse Monoclonal EDEM2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. C20orf31 - chromosome 20 open reading frame 31
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EDEM2 Antibody (2E4) [H00055741-M01] - Analysis of EDEM2 expression in transfected 293T cell line by C20orf31 monoclonal antibody (M01), clone 2E4.Lane 1: EDEM2 transfected lysate(64.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: EDEM2 Antibody (2E4) [H00055741-M01] - Detection limit for recombinant GST tagged C20orf31 is approximately 30ng/ml as a capture antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: EDEM2 Antibody (2E4) [H00055741-M01] - Detection limit for recombinant GST tagged EDEM2 is 0.1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: EDEM2 Antibody (2E4) [H00055741-M01] - Analysis of EDEM2 transfected lysate using anti-EDEM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EDEM2 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EDEM2 Antibody (2E4) [H00055741-M01] - Analysis of monoclonal antibody to C20orf31 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 1.5 ug/ml.
