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LearnPA5-120535
antibody from Invitrogen Antibodies
Targeting: EDEM2
bA4204.1, C20orf31, C20orf49, FLJ10783
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PA5-120535 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- EDEM2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive test controls include: A375, HepG2.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.96 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of EDEM2 in various cell lines using a EDEM2 Polyclonal antibody (Product # PA5-120535) at a dilution of 1:3,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 90s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of EDEM2 in L929 cells using a EDEM2 Polyclonal antibody (Product # PA5-120535). Blue: DAPI for nuclear staining.
