857505

antibody from BioLegend

Targeting: MFF C2orf33, GL004

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

857505

Provider

BioLegend

Product name

Biotin anti-MFF

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat

Host

Mouse

Conjugate

Biotin

Antibody clone number

N382/14

Vial size

25 µg

Concentration

0.50 mg/mL

Storage

2°C-8°C

Handling

Ambient RT

Immunocytochemistry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

ICC staining of Biotin anti-MFF antibody (clone N328/14) on HeLa cells. The cells were fixed with 4% PFA, permeabilized with 0.1% Triton X-100, and blocked with 2% normal goat serum and 0.02% bovine serum albumin. The cells were then stained with 5.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) for 1 hour at room temperature. The cells were counterstained with Flash Phalloidin™ Green 488 (Cat. No. 424201) and DAPI to visualize actin filaments and nuclei, respectively. The image was captured with a 60X objective. Scale bar: 20 µm

Conjugate

Biotin