Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
Reference
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- Product number
- NB100-60439 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-60439, RRID:AB_905423
- Product name
- Rabbit Polyclonal PALB2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Decreased glutathione biosynthesis contributes to EGFR T790M-driven erlotinib resistance in non-small cell lung cancer.
APRIN is a cell cycle specific BRCA2-interacting protein required for genome integrity and a predictor of outcome after chemotherapy in breast cancer.
Li H, Stokes W, Chater E, Roy R, de Bruin E, Hu Y, Liu Z, Smit EF, Heynen GJ, Downward J, Seckl MJ, Wang Y, Tang H, Pardo OE
Cell discovery 2016;2:16031
Cell discovery 2016;2:16031
APRIN is a cell cycle specific BRCA2-interacting protein required for genome integrity and a predictor of outcome after chemotherapy in breast cancer.
Brough R, Bajrami I, Vatcheva R, Natrajan R, Reis-Filho JS, Lord CJ, Ashworth A
The EMBO journal 2012 Mar 7;31(5):1160-76
The EMBO journal 2012 Mar 7;31(5):1160-76
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PALB2 Antibody [NB100-60439] - Detection of Human PALB2 on HeLa whole cell lysate using NB100-60439. PALB2 was also immunoprecipitated by rabbit anti-PALB2 antibody NB100-60440.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PALB2 Antibody [NB100-60439] - Whole cell lysate (15 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PALB2 antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PALB2 Antibody [NB100-60439] - Detection of human PALB2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PALB2 antibody NB100-60439 used for IP at 3 ug per reaction. PALB2 was also immunoprecipitated by rabbit anti-PALB2 antibody NB100-60440. For blotting immunoprecipitated PALB2, NB100-60439 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.