11825-1-AP

antibody from Invitrogen Antibodies

Targeting: RAG2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

11825-1-AP

Provider

Invitrogen Antibodies

Product name

RAG2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: VADCLPCVF LVDFEFGCAT SYILPELQDG LSFHVSIAKN DTIYILGGHS LANNIRPANL YRIRVDLPLG SPAVNCTVLP GGISVSSAIL TQTNNDEFVI VGGYQLENQK RMICNIISLE DNKIEIREME TPDWTPDIKH SKIWFGSNTG NGTVFLGIPG DNKQVVSEGF YFYMLKCAED DTNEEQTTFT NSQTSTEDPG DSTPFEDSEE FCFSAEANSF DGDDEFDTYN EDDEEDESET GYWITCCPTC DVDINTWVPF YSTELNKPAM IYCSHGDGHW VHAQCMDLAE RTLIHLSAGS NKYYCNEHVE IARALHTPQR VLPLKKPPMK SLRKKGSGKI LTPAKKSFLR RLFD (175-527 aa encoded by B C022397)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A375 cells were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11825-1-AP (RAG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using RAG2 antibody 11825-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human lymphoma using 11825-1-AP (RAG2 antibody) at dilution of 1:50 (under 10x lens).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 HeLa cells were stained with 0.2ug RAG2 antibody (11825-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-RAG2 (IP:11825-1-AP, 4ug; Detection:11825-1-AP 1:300) with A375 cells lysate 3600ug.