PA5-30445

antibody from Invitrogen Antibodies

Targeting: RASA3 GAP1IP4BP, GAPIII

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-30445

Provider

Invitrogen Antibodies

Product name

RASA3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: SK-N-SH, IMR32, SK-N-AS, mouse brain.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RASA3 using 30 µg of IMR32 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a RASA3 polyclonal antibody (Product # PA5-30445) at a dilution of 1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RASA3 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a RASA3 Polyclonal Antibody (Product # PA5-30445) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of RASA3 was performed by separating 50 µg of Mouse tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a RASA3 Polyclonal Antibody (Product # PA5-30445) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RASA3 Polyclonal Antibody detects RASA3 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RASA3 protein stained by RASA3 Polyclonal Antibody (Product # PA5-30445) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

RASA3 Polyclonal Antibody detects RASA3 protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RASA3 protein stained by RASA3 Polyclonal Antibody (Product # PA5-30445) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using RASA3 (Product # PA5-30445) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.