H00007323-M01

antibody from Abnova Corporation

Targeting: UBE2D3 UbcH5C

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007323-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007323-M01, RRID:AB_425730

Product name

UBE2D3 monoclonal antibody (M01), clone 4C1-1E3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant UBE2D3.

Antigen sequence

MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQA
TIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFT
TRIYHPNINSNGSICLDILRSQWSPALTISKVLLS
ICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISRE
WTQKYAM


Isotype

IgG

Antibody clone number

4C1-1E3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

UBE2D3 monoclonal antibody (M01), clone 4C1-1E3 Western Blot analysis of UBE2D3 expression in Jurkat ( Cat # L017V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of UBE2D3 expression in transfected 293T cell line by UBE2D3 monoclonal antibody (M01), clone 4C1-1E3.Lane 1: UBE2D3 transfected lysate(16.7 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged UBE2D3 is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol