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- Antibody Data
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- References [1]
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- Western blot [3]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00011216-M04 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00011216-M04, RRID:AB_2225592
- Product name
- Mouse Monoclonal AKAP10 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. AKAP10 - A kinase (PRKA) anchor protein 10
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references D-AKAP2 interacts with Rab4 and Rab11 through its RGS domains and regulates transferrin receptor recycling.
Eggers CT, Schafer JC, Goldenring JR, Taylor SS
The Journal of biological chemistry 2009 Nov 20;284(47):32869-80
The Journal of biological chemistry 2009 Nov 20;284(47):32869-80
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AKAP10 Antibody (8C10) [H00011216-M04] - Western blot analysis of AKAP10 over-expressed 293 cell line. Lane 2 cotransfected with AKAP10 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00011216-M04. GAPDH (36.1 kDa) used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AKAP10 Antibody (8C10) [H00011216-M04] - Analysis of AKAP10 expression in transfected 293T cell line by AKAP10 monoclonal antibody (M04), clone 8C10. Lane 1: AKAP10 transfected lysatE (73.8 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AKAP10 Antibody (8C10) [H00011216-M04] - Analysis of AKAP10 expression in Raw 264.7.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: AKAP10 Antibody (8C10) [H00011216-M04] - Detection limit for recombinant GST tagged AKAP10 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AKAP10 Antibody (8C10) [H00011216-M04] - Analysis of monoclonal antibody to AKAP10 on formalin-fixed paraffin-embedded human testis. Antibody concentration 0.5 ug/ml
