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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [3]
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- Product number
- NBP2-24912 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Bax inhibitor 1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Unfolded protein response-induced dysregulation of calcium homeostasis promotes retinal degeneration in rat models of autosomal dominant retinitis pigmentosa.
Shinde V, Kotla P, Strang C, Gorbatyuk M
Cell death & disease 2016 Feb 4;7(2):e2085
Cell death & disease 2016 Feb 4;7(2):e2085
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bax inhibitor 1 Antibody (20F565) [NBP2-24912] - analysis of Bi-1 with this antibody. Lane 1: in vitro translated human Bi-1 protein. Lanes 2 and 3: IP/WB of Bi-1 protein enriched in the transgenic mouse brain (Lane 2) or wildtype brain (Lane 3). IP was done with a polyclonal Bi-1 antibody, followed by WB with this antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Bax inhibitor 1 Antibody (20F565) [NBP2-24912] - analysis of Bi-1 using Bi-1 antibody at 5 ug/ml: A) overview of stained human and mouse TMA, B-C) immunoreactivity in human archicortex (positive neurons of hippocampal gyrus) at 200X and 400X, D-E) strong immunoreactivity in neurons of human dorsal root ganglion.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Bax inhibitor 1 Antibody (20F565) [NBP2-24912] - Formalin-fixed, paraffin-embedded human brain stained with Bi-1 antibody at 0.5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Bax inhibitor 1 Antibody (20F565) [NBP2-24912] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human liver cancer using 5 ug/ml concentration of Bax inhibitor 1 antibody (clone 20F565). Cytoplasmic immuno-positivity of Bax inhibitor 1 (TMBIM6) was observed in the hepatis cancer cells whereas the cells of tumor stroma did not develop any staining [10X Magnification]
