MA1-41108

antibody from Invitrogen Antibodies

Targeting: TMBIM6 BAXI1, BI-1, TEGT

Provider product page for MA1-41108

Western blot

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [2]
Comments [0]
Validations
Immunohistochemistry [4]
Other assay [2]

Submit

Product number: MA1-41108 - Provider product page
Provider: Invitrogen Antibodies
Product name: Bi-1 Monoclonal Antibody (20F430)
Antibody type: Monoclonal
Antigen: Synthetic peptide
Description: Epitope mapping indicates that the antibody recognizes an epitope in the N-terminal region of Bi-1.
Reactivity: Human, Mouse, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: 20F430
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

TMBIM6 protein structure - MA1-41108 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of Bi-1 in formalin-fixed, paraffin-embedded human adenocarcinoma of the rectum. Samples were incubated in Bi-1 monoclonal antibody (Product # MA1-41108) using a dilution of 5 µg/mL followed by peroxidase-conjugate and DAB chromogen. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of Bi-1 in formalin-fixed paraffin-embedded tissue section of human liver cancer. Samples were incubated in Bi-1 monoclonal antibody (Product # MA1-41108) using a dilution of 5 µg/mL. Intense cytoplasmic immune-positivity of Bax inhibitor 1 (TMBIM6) was observed in the hepatis cancer cells [10X Magnification].

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of Bi-1 in formalin-fixed, paraffin-embedded tissue sections. Samples were incubated in Bi-1 monoclonal antibody (Product # MA1-41108) using a dilution of 5 µg/mL. A: normal human lung alveoli with positive lung macrophages. B: normal mouse cartilage with positive chondrocytes. Hematoxylin-eosin counterstain.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of Bi-1 in formalin-fixed, paraffin-embedded normal human tissue sections. Samples were incubated in Bi-1 monoclonal antibody (Product # MA1-41108) using a dilution of 5 µg/mL. A: liver. B: skin. Hematoxylin-eosin counterstain.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1 TMBIM6 is downregulated in fatty degenerated and in aged human and mice livers. The liver was isolated from young and old WT mice and subjected to RNA-sequencing. (A) Hierarchical clustering of genes expressed in young and old WT mice. (B) Enrichment analysis results of up/down-regulated differentially expressed genes (DEGs) in old-aged mice compared to young mice. (C-D) Human normal and fat-degenerated liver sections were selected from tissue microarray (LV1201a and BC03116a) and analyzed for H&E and immunohistochemistry using TMBIM6 antibody. H&E images were adopted from Biomax. The bar graph (right) shows the average TMBIM6 expression score (LV1201a: normal = 14, fatty = 15; BC03116a: normal = 21, fatty = 9). Scale bar: 50 mum. (E) Normal tissues and fat-degenerated tissues were selected from young (50 years) (selected as explained in the method section), and TMBIM6 expression was analyzed by immunohistochemistry using a specific antibody. Bar graph (below) shows the average TMBIM6 expression (total young normal = 81, total old normal = 21, young fatty = 14, and old fatty = 10). Scale bar: 50 mum. (F) TMBIM6 mRNA expression was analyzed by real-time PCR in different age groups of wild-type mice. (G) Immunohistochemistry of the expression of TMBIM6 in liver sections of young and old WT and TMBIM6 -/- mice. The graph (right) shows the average TMBIM6 expression score (n = 6 per group). Note: TMBIM6 -/- mice liver sections were used to vali

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 6 Aging decreases TMBIM6 expression, induces aberrant UPR expression, S-nitrosylation, and sulfonation of IRE1alpha in human liver. (A) TMBIM6 mRNA expression and (B) protein expression were analyzed by real-time PCR and immunohistochemistry respectively in young (n = 7) and old human liver tissues (n = 15). (C) Western blotting was performed to check the expression of ER stress response elements p-IRE1, IRE1, GRP78, and CHOP. GAPDH was used as a loading control. Densitometry analysis is shown in the graph (right). (D) The mRNA expression of sXBP1 was analyzed by real-time PCR. (E) The mRNA expression of XBP1 target genes such as EDEM1 and ERDJ4 were measured by real-time PCR. (F) The mRNA expression of the IRE1alpha-dependent decay (RIDD) target genes such as CYP2E1 and GEMIN5 were examined in the human patient liver samples and analyzed by real-time PCR. (G) Western blotting was performed to check the expression of ER stress-apoptosis-related proteins such as caspase 12, caspase 7, p-JNK, and JNK. GAPDH was used as a loading control. (H) Dihydroethidium (DHE) and Dihydrorhodamine-123 (DHR-123) fluorescence staining were performed in liver sections of young and old patients using confocal microscopy. Red fluorescence indicates DHE positive cells, green fluorescence indicates DHR-123 positive cells, and blue fluorescence indicates DAPI for nuclear staining. The bar graph (right) shows the intensity (n = 7-9 for DHE, n = 5-11 for DHR-123). Magnification: 40x, Scale bar: 2