MA1-41678

antibody from Invitrogen Antibodies

Targeting: TMBIM6 BAXI1, BI-1, TEGT

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-41678

Provider

Invitrogen Antibodies

Product name

Bi-1 Monoclonal Antibody (20F248)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

MA1-41678 is derived from a mixture of three synthetic peptides corresponding to the N-terminal, internal and C-terminal regions of human B1.  Epitope mapping indicates that the antibody recognizes an epitope in the N-terminal region of Bi-1.

Reactivity

Human

Host

Mouse

Isotype

IgM

Antibody clone number

20F248

Vial size

100 µL

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Bi-1 in A) A549 and B) human lung lysate. Samples were incubated in Bi-1 monoclonal antibody (Product # MA1-41678) using a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Bax inhibitor 1 in HeLa cells. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti Bax inhibitor 1 (20F248) Antibody (Product # MA1-41678) at a 1:200 dilution overnight at 4C and detected with an anti-mouse IgM Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin (Red) at a 1:200 dilution. Nuclei wre counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of Bi-1 in THP-1 cells. Samples were incubated in Bi-1 monoclonal antibody (Product # MA1-41678) using a dilution of 1.0 µg/mL for 30 minutes at room temperature followed by Dylight488-conjugated anti-mouse secondary antibody. Antibody (blue) and a matched isotype control NBP2-27284 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin.