H00006788-D01P

antibody from Abnova Corporation

Targeting: STK3 KRS1, MST2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006788-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006788-D01P, RRID:AB_1710164

Product name

STK3 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human STK3 protein.

Antigen sequence

MEQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGE
GSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKE
ISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAG
SVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFM
RKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTM
AKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGIT
SIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPE
LWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKN
AKPVSILRDLITEAMEIKAKRHEEQQRELEEEEEN
SDEDELDSHTMVKTSVESVGTMRATSTMSEGAQTM
IEHNSTMLESDLGTMVINSEDEEEEDGTMKRNATS
PQVQRPSFMDYFDKQDFKNKSHENCNQNMHEPFPM
SKNVFPDNWKVPQDGDFDFLKNLSLEELQMRLKAL
DPMMEREIEELRQRYTAKRQPILDAMDAKKRRQQN
F


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of STK3 expression in transfected 293T cell line (H00006788-T03) by STK3 MaxPab polyclonal antibody.Lane 1: STK3 transfected lysate(56.30 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

STK3 MaxPab rabbit polyclonal antibody. Western Blot analysis of STK3 expression in A-431.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to STK3 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between STK3 and CASP3. HeLa cells were stained with anti-STK3 rabbit purified polyclonal 1:1200 and anti-CASP3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)