Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- 12097-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- STK3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MEQPPAPKS KLKKLSEDSL TKQPEEVFDV LEKLGEGSYG SVFKAIHKES GQVVAIKQVP VESDLQEIIK EISIMQQCDS PYVVKYYGSY FKNTDLWIVM EYCGAGSVSD IIRLRNKTLI EDEIATILKS TLKGLEYLHF MRKIHRDIKA GNILLNTEGH AKLADFGVAG QLTDTMAKRN TVIGTPFWMA PEVIQEIGYN CVADIWSLGI TSIEMAEGKP PYADIHPMRA IFMIPTNPPP TFRKPELWSD DFTDFVKKCL VKNPEQRATA TQLLQHPFIK NAKPVSILRD LITEAMEIKA KRHEEQQ (1-306 aa encoded by BC010640)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.14 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 12097-1-AP (STK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A375 cells were subjected to SDS PAGE followed by western blot with 12097-1-AP (STK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using STK3 antibody (Product # 12097-1-AP) at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudo color = DAPI (fluorescent DNA dye).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( 4% PFA ) fixed HeLa cells using 12097-1-AP (STK3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human ovary tumor using 12097-1-AP (STK3 antibody) at dilution of 1:50 (under 10x lens).