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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
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Validation data
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- Product number
- GTX111494 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111494, RRID:AB_11166238
- Product name
- STK3 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: THP-1 B: NCI-H929 7.5% SDS PAGE GTX111494 diluted at 1:1000
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- GeneTex (provider)
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- Experimental details
- Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: STK3 transfected 293T lysates 7.5% SDS PAGE GTX111494 diluted at 1:5000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- STK3 antibody detects STK3 protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 10% SDS-PAGE, and the membrane was blotted with STK3 antibody (GTX111494) diluted by 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- STK3 antibody immunoprecipitates STK3 protein in IP experiments.IP samples: HeLa whole cell extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of STK3 protein by 4 £gg STK3 antibody (GTX111494)7.5 % SDS-PAGEThe immunoprecipitated STK3 protein was detected by STK3 antibody (GTX111494) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
