Explore
Validate
LearnMA5-23521
antibody from Invitrogen Antibodies
Targeting: ZFP57
bA145L22, bA145L22.2, C6orf40, ZNF698
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-23521 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZFP57 Monoclonal Antibody (3I1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Recommended positive controls: 3xFlag-human ZFP57-transfected 293T cells.
- Antibody clone number
- 3I1
- Concentration
- 1 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZFP57 in non-transfected (-) and ZFP57 -transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 µg). Samples were separated by 7.5% SDS-PAGE and the membrane was probed with a ZFP57 monoclonal antibody (Product # MA5-23521) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ZFP57 was performed by separating 30 µg of non-transfected (-) and ZFP57 -transfected (+) (including 3xFlag-tag) 293T whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ZFP57 Monoclonal Antibody (3I1) (Product # MA5-23521) at a dilution of 1:5000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of ZFP57 was performed in Mock and transfected 293T cells fixed in 4% paraformaldehyde at RT for 15 min. Green: ZFP57 Monoclonal Antibody (3I1) (Product # MA5-23521) diluted at 1:2000. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
