NBP1-76573

antibody from Novus Biologicals

Targeting: SPTLC2 hLCB2a, KIAA0526, LCB2, LCB2A

ELISA

Immunocytochemistry

Antibody data

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Validation data

Product number: NBP1-76573 - Provider product page
Provider: Novus Biologicals
Proper citation: Novus Cat#NBP1-76573, RRID:AB_11023797
Product name: Rabbit Polyclonal Serine Palmitoyltransferase 2 Antibody
Antibody type: Polyclonal
Description: Peptide affinity purified. At least three isoforms of SPT2 are known to exist
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 0.1 mg
Concentration: 1 mg/ml
Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

SPTLC2 protein structure - NBP1-76573 shown in red.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Serine Palmitoyltransferase 2 Antibody [NBP1-76573] - 3T3 cell lysate with Serine Palmitoyltransferase 2 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Serine Palmitoyltransferase 2 Antibody [NBP1-76573] - Western Blot Validation in Mouse 3T3/NIH Cell Lysate. Loading: 15 ug of lysates per lane. Antibodies: Serine Palmitoyltransferase 2, NBP1-76573 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Serine Palmitoyltransferase 2 Antibody [NBP1-76573] - Independent Antibody Validation via Protein Expression Profile in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Serine Palmitoyltransferase 2, NBP1-76573 (1 ug/ml), Serine Palmitoyltransferase 2 from Company B (1 ug/ml), and beta-actin (1 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Serine Palmitoyltransferase 2 Antibody [NBP1-76573] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: Serine Palmitoyltransferase 2, NBP1-76573 (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Supportive validation

Submitted by: Novus Biologicals (provider)
Main image
Experimental details: Western Blot: Serine Palmitoyltransferase 2 Antibody [NBP1-76573] - Western Blot Validation in Human Brain Tissue Lysate. Loading: 15 ug of lysates per lane. Antibodies: Serine Palmitoyltransferase 2, NBP1-76573 (Lane 1: 0.5 ug/mL and Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.