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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-97027 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- C3orf31 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SLAFVYGSGV YRQAGPSSDQ KNAMLDFVFT VDDPVAWHSK NLKKNWSHYS FLKVLGPKII TSIQNNYGAG VYYNSLIMCN GRLIKYGVIS TNVLIEDLLN WNNLYIAGRL QKPVKIISVN EDVTLRSALD RNLKSAVTAA FLMLPESFSE EDLFIEIAGL SYSGDFRMVV GEDKTKVLNI VKPNIAHFRE LYGSILQENP QVVYKSQQGW LEIDKSPEGQ FTQLMTLPKT LQQQINHIMD PPGKNRDVEE TLFQVAHDPD CGDVVRLGLK KSVIYSSLKL HKMWKGWLRK TS; Positive Samples: MCF7, K-562, Jurkat; Cellular Location: Matrix side, Mitochondrion inner membrane, Peripheral membrane protein
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.76 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Solid-phase inclusion as a mechanism for regulating unfolded proteins in the mitochondrial matrix.
Ruan L, McNamara JT, Zhang X, Chang AC, Zhu J, Dong Y, Sun G, Peterson A, Na CH, Li R
Science advances 2020 Aug;6(32):eabc7288
Science advances 2020 Aug;6(32):eabc7288
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of C3orf31 in extracts of various cell lines using C3orf31 Polyclonal Antibody (Product # PA5-97027) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of C3orf31 in paraffin-embedded mouse stomach using C3orf31 Polyclonal Antibody (Product # PA5-97027) at a dilution of 1:200.
