MA5-27600

antibody from Invitrogen Antibodies

Targeting: SYT12 SRG1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27600

Provider

Invitrogen Antibodies

Product name

Anti-SYT12 Monoclonal Antibody (S277-7)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

S277-7

Vial size

100 µg

Concentration

1 mg/ml

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SYT12 in monkey COS cells transfected with GFP-tagged Synaptotagmin with 15 µg of sample. The sample was blocked with 2% BSA and 2% skim milk in TBST, incubated with SYT12 monoclonal antibody (Product # MA5-27600) using a dilution of 1:200 (16 hours at RT), followed by Goat Anti-Mouse HRP (1 hour at RT) at a dilution of 1:1000 and ECL development (6 min at RT). Samples were arranged as follows: Lane 1: Molecular Weight Ladder, Lane 2: Monkey COS cells transfected with GFP-tagged Synaptotagmin.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SYT12 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with SYT12 monoclonal antibody (Product # MA5-27600) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Synaptotagmin-12 Antibody, D) Merged image. Magnification: 60x.