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Learn11302-1-AP
antibody from Invitrogen Antibodies
Targeting: RPL4
L4
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 11302-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ribosomal protein L4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MACARPLIS VYSEKGESSG KNVTLPAVFK APIRPDIVNF VHTNLRKNNR QPYAVSELAG HQTSAESWGT GRAVARIPRV RGGGTHRSGQ GAFGNMCRGG RMFAPTKTWR RWHRRVNTTQ KRYAICSALA ASALPALVMS KGHRIEEVPE LPLVVEDKVE GYKKTKEAVL LLKKLKAWND IKKVYASQRM RAGKGKMRNR RRIQRRGPCI IYNEDNGIIK AFRNIPGITL LNVSKLNILK LAPGGHVGRF CIWTESAFRK LDELYGTWRK AASLKSNYNL PMHKMINTDL SRILKSPEIQ RALRAPRKKI HRRVLKKNPL KNLRIMLKLN PYAKTMRRNT ILRQARNHKL RVDKAAAAAA ALQAKSDEKA AVAGKKPVVG KKGKKAAVGV KKQKKPLVGK KAAATKKPAP EKKPAEKKPT TEEKKPAA (1-427 aa encoded by BC009888)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.18 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 11302-1-AP (ribosomal protein L4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 11302-1-AP (ribosomal protein L4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 11302-1-AP (ribosomal protein L4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Rat brain tissue were subjected to SDS PAGE followed by western blot with 11302-1-AP ( ribosomal protein L4 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using RPL4 antibody 11302-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lymphoma using 11302-1-AP (ribosomal protein L4 antibody) at dilution of 1:100 (under 10x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HeLa cells were intracellularly stained with 0.2 µg Anti-Human Ribosomal protein L4 (Product # 11302-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (red), or 0.2 µg Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ribosomal protein L4 (IP:11302-1-AP, 3ug; Detection:11302-1-AP 1:800) with K-562 cells lysate 6000ug.
