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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00000439-M03 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00000439-M03, RRID:AB_536962
- Product name
- Mouse Monoclonal ASNA1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ASNA1 - arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Asna1/TRC40 that mediates membrane insertion of tail-anchored proteins is required for efficient release of Herpes simplex virus 1 virions.
VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase.
A precursor-specific role for Hsp40/Hsc70 during tail-anchored protein integration at the endoplasmic reticulum.
Ott M, Marques D, Funk C, Bailer SM
Virology journal 2016 Oct 20;13(1):175
Virology journal 2016 Oct 20;13(1):175
VAPB/ALS8 interacts with FFAT-like proteins including the p97 cofactor FAF1 and the ASNA1 ATPase.
Baron Y, Pedrioli PG, Tyagi K, Johnson C, Wood NT, Fountaine D, Wightman M, Alexandru G
BMC biology 2014 May 29;12:39
BMC biology 2014 May 29;12:39
A precursor-specific role for Hsp40/Hsc70 during tail-anchored protein integration at the endoplasmic reticulum.
Rabu C, Wipf P, Brodsky JL, High S
The Journal of biological chemistry 2008 Oct 10;283(41):27504-13
The Journal of biological chemistry 2008 Oct 10;283(41):27504-13
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ASNA1 Antibody (2H3) [H00000439-M03] - Western blot analysis of ASNA1 over-expressed 293 cell line. Lane 2 cotransfected with ASNA1 Validated Chimera RNAi Lane 1 non-transfected control. Blot probed with H00000439-M03. GAPDH (36.1 kDa) used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ASNA1 Antibody (2H3) [H00000439-M03] - Analysis of ASNA1 expression in transfected 293T cell line by ASNA1 monoclonal antibody (M03), clone 2H3. Lane 1: ASNA1 transfected lysatE (38.8 KDa). Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ASNA1 Antibody (2H3) [H00000439-M03] - Analysis of ASNA1 expression in HeLa (Cat # L013V1).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: ASNA1 Antibody (2H3) [H00000439-M03] - Detection limit for recombinant GST tagged ASNA1 is approximately 0.03ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: ASNA1 Antibody (2H3) [H00000439-M03] - Analysis of monoclonal antibody to ASNA1 on HeLa cell. Antibody concentration 10 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ASNA1 Antibody (2H3) [H00000439-M03] - Analysis of monoclonal antibody to ASNA1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 0.5 ug/ml
