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Learn15450-1-AP
antibody from Invitrogen Antibodies
Targeting: GET3
ARSA-I, ASNA1, TRC40
Western blot Immunocytochemistry
Immunoprecipitation Immunohistochemistry Flow cytometry Other assayAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- 15450-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ASNA1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: MAAGVAGWG VEAEEFEDAP DVEPLEPTLS NIIEQRSLKW IFVGGKGGVG KTTCSCSLAV QLSKGRESVL IISTDPAHNI SDAFDQKFSK VPTKVKGYDN LFAMEIDPSL GVAELPDEFF EEDNMLSMGK KMMQEAMSAF PGIDEAMSYA EVMRLVKGMN FSVVVFDTAP TGHTLRLLNF PTIVERGLGR LMQIKNQISP FISQMCNMLG LGDMNADQLA SKLEETLPVI RSVSEQFKDP EQTTFICVCI AEFLSLYETE RLIQELAKCK IDTHNIIVNQ LVFPDPEKPC KMCEARHKIQ AKYLDQMEDL YEDFHIVKLP LLPHEVRGAD KVNTFSALLL EPYKPPSAQ (1-348 aa encoded by BC002651 )
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.23 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human heart tissue were subjected to SDS PAGE followed by western blot with 15450-1-AP (ASNA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15450-1-AP (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Human brain tissue were subjected to SDS PAGE followed by western blot with 15450-1-AP (ASNA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse brain tissue were subjected to SDS PAGE followed by western blot with 15450-1-AP (ASNA1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells, using ASNA1 antibody 15450-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using 15450-1-AP (ASNA1 antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer using 15450-1-AP (ASNA1 antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 HepG2 cells were stained with 0.2ug ASNA1 antibody (15450-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ASNA1 (IP:15450-1-AP, 4ug; Detection:15450-1-AP 1:1000) with mouse brain tissue lysate 4000ug.
