H00000439-M03

antibody from Invitrogen Antibodies

Targeting: GET3 ARSA-I, ASNA1, TRC40

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000439-M03

Provider

Invitrogen Antibodies

Product name

ASNA1 Monoclonal Antibody (2H3)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Sequence of this protein is as follows: PEQTTFICVC IAEFLSLYET ERLIQELAKC KIDTHNIIVN QLVFPDPEKP CKMCEARHKI QAKYLDQMED LYEDFHIVKL PLLPHEVRGA DKVNTFSALL LEPYKPPSAQ

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

2H3

Vial size

100 µg

Concentration

See Label

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ASNA1 over-expressed 293 cell line, cotransfected with ASNA1 Validated Chimera RNAi (Cat # H00000439-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ASNA1 monoclonal antibody (M03), clone 2H3 (Cat # H00000439-M03). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of ASNA1 expression in transfected 293T cell line by ASNA1 monoclonal antibody (M03), clone 2H3.Lane 1: ASNA1 transfected lysate(38.8 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ASNA1 monoclonal antibody (M03), clone 2H3 Western Blot analysis of ASNA1 expression in HeLa (Cat # L013V1).

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ASNA1 is approximately 0.03 ng/mL as a capture antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to ASNA1 on HeLa cell. Antibody concentration is 10 µg/mL.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to ASNA1 on formalin-fixed paraffin-embedded human testis. Antibody concentration is 0.5 µg/mL.