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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-51336 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TP53I11 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.4 mg/mL
- Storage
- -20°C
Submitted references High expression of PIG11 correlates with poor prognosis in gastric cancer.
Gu J, Zhang S, He X, Chen S, Wang Y
Experimental and therapeutic medicine 2021 Mar;21(3):249
Experimental and therapeutic medicine 2021 Mar;21(3):249
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of TP53I11 was performed by loading Jurkat cells lysates (40µg) on to a 8% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a TP53I11 antibody (Product # PA5-51336) at a 1/400 dilution for 1 second, followed by Goat anti rabbit IgG at 1/8000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TP53I11 in paraffin embedded Human liver cancer tissue using TP53I11 Polyclonal Antibody (Product # PA5-51336) at a 1:40 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of TP53I11 in paraffin embedded Human thyroid cancer tissue using TP53I11 Polyclonal Antibody (Product # PA5-51336) at a 1:40 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 Immunohistochemical images for the detection of PIG11 protein expression in GC and non-cancerous tissues in the present study. (A) In chronic gastritis tissues (n=42), (B) intestinal metaplasia tissues (n=80), (C) surgical margin tissues (n=60) and (D) low-grade intraepithelial neoplasia (n=56), weak expression was observed. (E) In high-grade intraepithelial neoplasia (n=72) and (F) GC tissues (n=480), positive expression was observed. PIG11 protein was high in cancerous tissues. Original magnifications of x400 (scale bar, 50 um) and x40 (scale bar, 500 um). PIG11, P53-induced gene 11; GC, gastric cancer.
