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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NBP2-22321 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal GOLGB1/Giantin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references The Role of Alcohol-Induced Golgi Fragmentation for Androgen Receptor Signaling in Prostate Cancer.
Post-ER Stress Biogenesis of Golgi Is Governed by Giantin.
Functional disruption of the Golgi apparatus protein ARF1 sensitizes MDA-MB-231 breast cancer cells to the antitumor drugs Actinomycin D and Vinblastine through ERK and AKT signaling.
Giantin Is Required for Post-Alcohol Recovery of Golgi in Liver Cells.
Manca S, Frisbie CP, LaGrange CA, Casey CA, Riethoven JM, Petrosyan A
Molecular cancer research : MCR 2019 Jan;17(1):225-237
Molecular cancer research : MCR 2019 Jan;17(1):225-237
Post-ER Stress Biogenesis of Golgi Is Governed by Giantin.
Frisbie CP, Lushnikov AY, Krasnoslobodtsev AV, Riethoven JM, Clarke JL, Stepchenkova EI, Petrosyan A
Cells 2019 Dec 13;8(12)
Cells 2019 Dec 13;8(12)
Functional disruption of the Golgi apparatus protein ARF1 sensitizes MDA-MB-231 breast cancer cells to the antitumor drugs Actinomycin D and Vinblastine through ERK and AKT signaling.
Luchsinger C, Aguilar M, Burgos PV, Ehrenfeld P, Mardones GA
PloS one 2018;13(4):e0195401
PloS one 2018;13(4):e0195401
Giantin Is Required for Post-Alcohol Recovery of Golgi in Liver Cells.
Casey CA, Thomes P, Manca S, Petrosyan A
Biomolecules 2018 Nov 16;8(4)
Biomolecules 2018 Nov 16;8(4)
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GOLGB1/Giantin Antibody [NBP2-22321] - Whole cell lysate (50 ug) from 293T, HeLa, and Jurkat cells. NBP2-22321 used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: GOLGB1/Giantin Antibody [NBP2-22321] - HeLa cell lysate sample treated with DMSO was run on 8% SDS-PAGE. Both giantin monomer and dimer are indicated. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: GOLGB1/Giantin Antibody [NBP2-22321] - Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: NBP2-22321 used for IP at 6 ug/mg lysate. GOLGB1 was also immunoprecipitated by rabbit anti-GOLGB1 antibodies NBP2-22322 and NBP2-22323. For blotting immunoprecipitated GOLGB1, NBP2-22321 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
