PA5-142688

antibody from Invitrogen Antibodies

Targeting: SDF4 Cab45

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-142688

Provider

Invitrogen Antibodies

Product name

SDF4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Additional Information: This antibody is expected to recognize both reported isoforms NP_057631.2; and NP_057260.3.

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of SDF4 in Jurkat (A), HeLa (B) and U251 (C) cell lysate (35 µg protein in RIPA buffer) using SDF4 Polyclonal Antibody (Product # PA5-142688) at 0.1 µg/mL. Detected by chemiluminescence.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of SDF4 in U2OS cells using SDF4 Polyclonal Antibody (Product # PA5-142688). Cells were fixed with paraformaldehyde, permeabilized with 0.15% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing Golgi and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of SDF4 in paraffin embedded Human Kidney using SDF4 Polyclonal Antibody (Product # PA5-142688) at 5 µg/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of SDF4 in A431 cells (blue line) using SDF4 Polyclonal Antibody (Product # PA5-142688). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton, and incubated with the primary antibody for 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.