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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-30539 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HOOK2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, HCT116, mouse liver, HEK293T.
- Concentration
- 1 mg/mL
Submitted references LRGUK1 is part of a multiprotein complex required for manchette function and male fertility.
Okuda H, DeBoer K, O'Connor AE, Merriner DJ, Jamsai D, O'Bryan MK
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Mar;31(3):1141-1152
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 Mar;31(3):1141-1152
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HOOK2 Polyclonal Antibody (Product # PA5-30539). Sample (50 µg of whole cell lysate). Lane A: mouse liver . 7.5% SDS PAGE. HOOK2 Polyclonal Antibody (Product # PA5-30539) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HOOK2 Polyclonal Antibody (Product # PA5-30539). Sample (30 µg of whole cell lysate). Lane A: HCT116. 7.5% SDS PAGE. HOOK2 Polyclonal Antibody (Product # PA5-30539) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of HOOK2 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: HOOK2 Polyclonal Antibody (Product # PA5-30539) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of HOOK2 was performed in paraffin-embedded mouse duodenum tissue using HOOK2 Polyclonal Antibody (Product # PA5-30539) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of HOOK2 was performed in paraffin-embedded mouse testis tissue using HOOK2 Polyclonal Antibody (Product # PA5-30539) at a dilution of 1:500.
