Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- PA5-117366 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- POLR2K Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Centrifuge briefly prior to opening.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.61 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Western blot analysis of POLR2K in SH-SY5Y whole cell and nuclear extracts (30 µg). Samples were separated with 15% SDS-PAGE and incubated in POLR2K polyclonal antibody (Product # PA5-117366) using a dilution of 1:1,000, followed by HRP-conjugated anti-rabbit IgG. Detection was performed with Trident ECL plus-Enhanced.