HPA053682

antibody from Atlas Antibodies

Targeting: SPAG1 CILD28, CT140, FLJ32920, HSD-3.8, SP75, TPIS

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA053682

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA053682, RRID:AB_2682229

Product name

Anti-SPAG1

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

KKTPRDYAEWDKFDVEKECLKIDEDYKEKTVIDKS
HLSKIETRIDTAGLTEKEKDFLATREKEKGNEAFN
SGDYEEAVMYYT


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human testis and prostate tissues using Anti-SPAG1 antibody. Corresponding SPAG1 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human endometrium shows strong cytoplasmic positivity in ciliated glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows low expression as expected.

Sample type

HUMAN