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LearnPA5-49729
antibody from Invitrogen Antibodies
Targeting: SPAG1
CILD28, CT140, FLJ32920, HSD-3.8, SP75, TPIS
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-49729 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPAG1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody detects endogenous level of total SPAG1 protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SPAG1 was performed by loading Human colon tissue lysate (40µg) on to a 8% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a SPAG1 antibody (Product # PA5-49729) at a 1/600 dilution for 2 minutes, followed by Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SPAG1 was performed by loading Human colon tissue lysate (40µg) on to a 8% SDS-PAGE gel. Proteins were transferred to a membrane and the membrane was probed with a SPAG1 antibody (Product # PA5-49729) at a 1/600 dilution for 2 minutes, followed by Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of SPAG1 in paraffin-embedded human ovarian cancer tissue using (left) SPAG1 Polyclonal Antibody (Product # PA5-49729) at a 1:25 dilution and (right) is treated with the synthetic peptide.
