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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Western blot [2]
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- Product number
- H00085377-B01P - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00085377-B01P, RRID:AB_1678047
- Product name
- MICAL-L1 purified MaxPab mouse polyclonal antibody (B01P)
- Antibody type
- Polyclonal
- Description
- Mouse polyclonal antibody raised against a full-length human MICAL-L1 protein.
- Antigen sequence
MAGPRGALLAWCRRQCEGYRGVEIRDLSSSFRDGL
AFCAILHRHRPDLLDFDSLSKDNVFENNRLAFEVA
EKELGIPALLDPNDMVSMSVPDCLSIMTYVSQYYN
HFCSPGQAGVSPPRKGLAPCSPPSVAPTPVEPEDV
AQGEELSSGSLSEQGTGQTPSSTCAACQQHVHLVQ
RYLADGRLYHRHCFRCRRCSSTLLPGAYENGPEEG
TFVCAEHCARLGPGTRSGTRPGPFSQPKQQHQQQL
AEDAKDVPGGGPSSSAPAGAEADGPKASPEARPQI
PTKPRVPGKLQELASPPAGRPTPAPRKASESTTPA
PPTPRPRSSLQQENLVEQAGSSSLVNGRLHELPVP
KPRGTPKPSEGTPAPRKDPPWITLVQAEPKKKPAP
LPPSSSPGPPSQDSRQVENGGTEEVAQPSPTASLE
SKPYNPFEEEEEDKEEEAPAAPSLATSPALGHPES
TPKSLHPWYGITPTSSPKTKKRPAPRAPSASPLAL
HASRLSHSEPPSATPSPALSVESLSSESASQTAGA
ELLEPPAVPKSSSEPAVHAPGTPGNPVSLSTNSSL
ASSGELVEPRVEQMPQASPGLAPRTRGSSGPQPAK
PCSGATPTPLLLVGDRSPVPSPGSSSPQLQVKSSC
KENPFNRKPSPAASPATKKATKGSKPVRPPAPGHG
FPLIKRKVQADQYIPEEDIHGEMDTIERRLDALEH
RGVLLEEKLRGGLNEGREDDMLVDWFKLIHEKHLL
VRRESELIYVFKQQNLEQRQADVEYELRCLLNKPE
KDWTEEDRAREKVLMQELVTLIEQRNAIINCLDED
RQREEEEDKMLEAMIKKKEFQREAEPEGKKKGKFK
TMKMLKLLGNKRDAKSKSPRDKS- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Diacylglycerol kinase α regulates tubular recycling endosome biogenesis and major histocompatibility complex class I recycling.
Rab35 promotes the recruitment of Rab8, Rab13 and Rab36 to recycling endosomes through MICAL-L1 during neurite outgrowth.
Rab35 establishes the EHD1-association site by coordinating two distinct effectors during neurite outgrowth.
MICAL-L1 links EHD1 to tubular recycling endosomes and regulates receptor recycling.
Xie S, Naslavsky N, Caplan S
The Journal of biological chemistry 2014 Nov 14;289(46):31914-26
The Journal of biological chemistry 2014 Nov 14;289(46):31914-26
Rab35 promotes the recruitment of Rab8, Rab13 and Rab36 to recycling endosomes through MICAL-L1 during neurite outgrowth.
Kobayashi H, Etoh K, Ohbayashi N, Fukuda M
Biology open 2014 Aug 1;3(9):803-14
Biology open 2014 Aug 1;3(9):803-14
Rab35 establishes the EHD1-association site by coordinating two distinct effectors during neurite outgrowth.
Kobayashi H, Fukuda M
Journal of cell science 2013 Jun 1;126(Pt 11):2424-35
Journal of cell science 2013 Jun 1;126(Pt 11):2424-35
MICAL-L1 links EHD1 to tubular recycling endosomes and regulates receptor recycling.
Sharma M, Giridharan SS, Rahajeng J, Naslavsky N, Caplan S
Molecular biology of the cell 2009 Dec;20(24):5181-94
Molecular biology of the cell 2009 Dec;20(24):5181-94
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- MICALL1 MaxPab polyclonal antibody. Western Blot analysis of MICALL1 expression in HepG2.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of MICALL1 expression in transfected 293T cell line (H00085377-T01) by MICALL1 MaxPab polyclonal antibody.Lane 1: MICAL-L1 transfected lysate(94.93 KDa).Lane 2: Non-transfected lysate.
