NBP2-70017

antibody from Novus Biologicals

Targeting: MICALL1 KIAA1668, MICAL-L1, MIRAB13

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

NBP2-70017

Provider

Novus Biologicals

Product name

Rabbit Polyclonal MICALL1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: MICALL1 Antibody [NBP2-70017] - Total protein from HEK293, A431 and U2OS was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-MICALL1 in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: MICALL1 Antibody [NBP2-70017] - IHC analysis of a formalin fixed paraffin embedded tissue section of the human testis using 1:200 dilution of MICALL1 antibody (NBP2-70017). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. The antibody generated mainly a moderate whole cell with nuclear staining.