AF739

antibody from Novus Biologicals

Targeting: GDF9

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF739

Provider

Novus Biologicals

Product name

Goat Polyclonal GDF-9 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse GDF-9 in direct ELISAs and both mouse and hamster GDF-9 in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse (rm) GDF-1, rmGDF-5, rmGDF-6, rmGDF-8, and rmGDF-15 is observed.

Reactivity

Mouse, Hamster

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

GDF9 is transiently expressed in oocytes before follicle formation in the human fetal ovary and is regulated by a novel NOBOX transcript.

Bayne RA, Kinnell HL, Coutts SM, He J, Childs AJ, Anderson RA
PloS one 2015;10(3):e0119819

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Oocyte generation in adult mammalian ovaries by putative germ cells in bone marrow and peripheral blood.

Johnson J, Bagley J, Skaznik-Wikiel M, Lee HJ, Adams GB, Niikura Y, Tschudy KS, Tilly JC, Cortes ML, Forkert R, Spitzer T, Iacomini J, Scadden DT, Tilly JL
Cell 2005 Jul 29;122(2):303-15

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse and Hamster GDF-9 by Western Blot. Western blot shows lysates of human ovary tissue and CHO Chinese hamster ovary cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Hamster GDF-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF739) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GDF-9 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

GDF-9 in Mouse Ovary. GDF-9 was detected in perfusion fixed frozen sections of mouse ovary using Goat Anti-Mouse/Hamster GDF-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF739) at 5 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to developing oocytes. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.