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- Product number
- NBP2-61934 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP2-61934, RRID:AB_2801394
- Product name
- Mouse Monoclonal GDF-9 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: GDF-9 Antibody ([53/1]) - Azide and BSA Free [NBP2-61934] - Biological neutralizing capacity of 53/1 by ELISA. Biological neutralizing specificity of anti-GDF-9-53 with some members of the TGFbeta superfamily. Mouse MGC were cultured with human TGFbeta1 (0.5 ng/ml), human activin A (50 ng/ml), or mouse GDF-9 (40 ng/ml), either in the absence or presence of a high neutralizing dose of 40 ug/ml of mAb-GDF-9-53 or 40 ug/ml human IgG.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: GDF-9 Antibody ([53/1]) - Azide and BSA Free [NBP2-61934] - Clone 53/1 used to detect GDF-9 expression by ELISA. GDF-9 ELISA. A GDF-9 ELISA was developed to measure the amount of GDF-9 in HEK-293T conditioned medium. Recombinant mouse GDF-9 was used as a standard, and the specificity of the assay was assessed using a range of TGF-beta family members; wild-type human GDF-9, human GDF-9 L40V, human BMP-15/GDF-9B, human activin A, and human TGF-beta3. Dilutions of concentrated media from cells transfected with empty vector, pcDNA3.1 (r), were included as controls. The ELISA has a specificity of less than 0.1%, with a sensitivity of 0.2 ng/mL. Values represent mean +/- SEM in duplicate, from a representative experiment.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: GDF-9 Antibody ([53/1]) - Azide and BSA Free [NBP2-61934] - Clone 53/1 used to detect GDF-9 expression in oocyte-medium via western blot. GDF-9 immunoblot analysis. A) Antibody specificity for GDF-9 and (B) detection of oocyte-secreted GDF-9. The recombinant proteins indicated (mGDF-9 = mouse GDF-9, oBMP-15/GDF-9B = ovine BMP-15/GDF-9B) and oocyte extract or oocyte-conditioned medium were subjected to SDS-PAGE immunoblotting with the anti-GDF-9 monoclonal antibody and detected using either ECL (A; recombinant proteins) or ECL Advance (B; oocyte products). The 57-kDa band is the GDF-9 proprotein while the 17.5-kDa band represents the mature GDF-9 monomer. 293H = control; conditioned medium from the untransfected human embryonic kidney 293H parent cell line.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: GDF-9 Antibody ([53/1]) - Azide and BSA Free [NBP2-61934] - Clone 53/1 used to detect GDF-9 expression in oocyte-medium by western blot. Samples were detected under reducing conditions using GDF-9 mAb53 specific for the mature domain. The 60-kDa GDF-9 precursor and 20-kDa mature monomer are shown.
