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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- AP52904PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52904PU-N, RRID:AB_11144951
- Product name
- anti CARD4 / NOD1 (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 930-953aa) of human CARD4 / NOD1
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis in HL-60, CEM cell line lysates (35ug/lane) using CARD4 / NOD1 antibody Cat.-No. AP52904PU-N (C-term). This demonstrates this antibody detected the NOD1 protein (arrow).
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis in mouse bladder tissue lysates (35ug/lane) using CARD4 / NOD1 antibody Cat.-No. AP52904PU-N (C-term). This demonstrates this antibody detected the NOD1 protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of NOD1 in HepG2 cell using CARD4 / NOD1 antibody Cat.-No. AP52904PU-N (C-term), followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in human pancreas tissue (Formalin-fixed, Paraffin-embedded) using CARD4 / NOD1 antibody Cat.-No. AP52904PU-N (C-term), followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of this antibody for IHC. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram) using CARD4 / NOD1Â antibody Cat.-No. AP52904PU-NÂ (C-term), followed by FITC-conjugated secondary antibodies.
