936302

antibody from BioLegend

Targeting: CRY2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

936302

Provider

BioLegend

Product name

Purified anti-CRY2

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Rat

Conjugate

Unconjugated

Antibody clone number

W17237A

Vial size

100 µg

Concentration

0.50 mg/mL

Storage

2°C-8°C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Whole cell extracts (15 µg protein) from NIH/3T3 cells transfected with non-targeting control siRNA (siCON) or siRNA targeting CRY2 (siCRY2) were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) of purified anti-CRY2 antibody (clone W17237A) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.

Conjugate

Unconjugated