Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-13125 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CRY2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Diurnal oscillations of endogenous H(2)O(2) sustained by p66(Shc) regulate circadian clocks.
Core circadian clock gene expression in human dental pulp-derived cells in response to L-mimosine, hypoxia and echinomycin.
Pei JF, Li XK, Li WQ, Gao Q, Zhang Y, Wang XM, Fu JQ, Cui SS, Qu JH, Zhao X, Hao DL, Ju D, Liu N, Carroll KS, Yang J, Zhang EE, Cao JM, Chen HZ, Liu DP
Nature cell biology 2019 Dec;21(12):1553-1564
Nature cell biology 2019 Dec;21(12):1553-1564
Core circadian clock gene expression in human dental pulp-derived cells in response to L-mimosine, hypoxia and echinomycin.
Janjić K, Kurzmann C, Moritz A, Agis H
European journal of oral sciences 2018 Aug;126(4):263-271
European journal of oral sciences 2018 Aug;126(4):263-271
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using a Cry2 polyclonal antibody (Product # PA5-13125) in mouse liver tissue and HepG2 cell lysates (35 µg per lane).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a Cry2 polyclonal antibody (Product # PA5-13125), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Core clock gene mRNA and protein levels under normoxia, L-mimosine (L- MIM ) and hypoxia change during the observation period. Dental pulp-derived cells ( DPC ) in two-dimensional (2D) monolayer cultures were serum-starved and afterwards treated with L- MIM or hypoxia. mRNA (A-D) and protein (E-I) levels of circadian locomotor output cycles kaput ( CLOCK ), cryptochrome circadian regulator 1 and 2 ( CRY 1 and CRY 2, respectively) and period circadian regulator 3 ( PER 3) were measured in a 4-h interval over 48 h by quantitative PCR ( qPCR ) and western blotting, respectively. mRNA levels are displayed relative to glyceraldehyde-3-phosphate dehydrogenase ( GAPDH ) and 0 h after serum starvation (A-D). GAPDH was used as reference protein (E-I). Experiments were conducted at least twice with DPC from two different donors ( n = 4).