AF3535

antibody from R&D Systems

Targeting: LGALS9 LGALS9A

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF3535

Provider

R&D Systems

Product name

Mouse Galectin-9 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects mouse Galectin-9 in direct ELISAs and Western blots. In Western blots, approximately 30% cross-reactivity with recombinant human (rh) Galectin-9 is observed. In direct ELISAs and Western blots, less than 2% cross-reactivity with rhGalectin-1, rhGalectin-2, rhGalectin-3, rhGalectin-7, rhGalectin-8, recombinant mouse (rm) Galectin-1, rmGalectin-3, rmGalectin-4, and rmGalectin-7 is observed.

Reactivity

Mouse

Host

Goat

Conjugate

Unconjugated

Antigen sequence

O08573


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Characterization of neutralizing antibodies reacting with the 213-224 amino-acid segment of human galectin-9.

Lhuillier C, Barjon C, Baloche V, Niki T, Gelin A, Mustapha R, Claër L, Hoos S, Chiba Y, Ueno M, Hirashima M, Wei M, Morales O, Raynal B, Delhem N, Dellis O, Busson P
PloS one 2018;13(9):e0202512

---

Galectin-9 Is Critical for Mucosal Adaptive Immunity through the T Helper 17-IgA Axis.

Liang CC, Li CS, Weng IC, Chen HY, Lu HH, Huang CC, Liu FT
The American journal of pathology 2018 May;188(5):1225-1235

---

Astrocyte galectin-9 potentiates microglial TNF secretion.

Steelman AJ, Li J
Journal of neuroinflammation 2014 Aug 27;11:144

---

TIM-3 does not act as a receptor for galectin-9.

Leitner J, Rieger A, Pickl WF, Zlabinger G, Grabmeier-Pfistershammer K, Steinberger P
PLoS pathogens 2013 Mar;9(3):e1003253

---

Galectin-9 protein is up-regulated in astrocytes by tumor necrosis factor and promotes encephalitogenic T-cell apoptosis.

Steelman AJ, Smith R 3rd, Welsh CJ, Li J
The Journal of biological chemistry 2013 Aug 16;288(33):23776-87

---

Endogenous galectin-1 and acute inflammation: emerging notion of a galectin-9 pro-resolving effect.

Iqbal AJ, Sampaio AL, Maione F, Greco KV, Niki T, Hirashima M, Perretti M, Cooper D
The American journal of pathology 2011 Mar;178(3):1201-9

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Galectin-9 in Mouse Splenocytes. Galectin-9 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Galectin-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3535) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Galectin-9 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Goat Anti-Mouse Galectin-9 Short Isoform Affinity-purified Polyclonal Antibody (Catalog # AF3535, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.